Pretreating Epoxy Thin Sections With Sodium Periodate Prior To Immunostaining

Preparation of thin epoxy resin sections from thick sections of paraffin-embedded material.

PROCEDURES The following have been used with success: haematoxylin and eosin, Perls's reaction for ferric iron, the periodic acid-Schiff reaction for carbohydrates, the alcian blue method for acid mucopolysaccharides, the Feulgen reaction for desoxyribonucleic acid, the periodic acid-hexamine silver method, Luxol fast blue for myelin, solochrome cyanine as a general stain, Heidenhain's iron hae...

Thin Sections

Knowledge of the thickness of sections is important for proper interpretation of electron micrographs. Therefore, the thicknesses of sections of n-butyl methacrylate polymer were determined by ellipsometry, and correlated with the color shown in reflected light. The results are: gray, thinner than 60 mmicro; silver, 60 to 90 mmicro; gold, 90 to 150 mmicro; purple, 150 to 190 mmicro; blue, 190 t...

Influence of Nitrogen Bases on Epoxidation of Cyclooctene with Sodium Periodate Catalysed by Manganese(III) Porphyrins

Effects of imidazoles and substituted pyridines and pure s-donors on the oxidation of cyclooctene with NaIO4 by 5,10,15,20-tetraphenylporphyrinato-manganese(III) acetate, Mn(TPP)OAc, and 5,10,15,20-Tetramesitylporphyrinato-manganese(III) acetate, Mn(TMP)OAc, catalysts in CH2Cl2-H2O solvent are studied. Coordination of the nitrogen donors to the Mn...

Educational Meteorite Thin Sections

Technical report Protocol for ultrarapid immunostaining of frozen sections

Rapid immunostaining of frozen sections within a tolerable time span would be very helpful for intraoperative diagnosis. A protocol was therefore established using the enhanced polymer one-step staining (EPOS) system (Dako) with antibodies against leucocyte common antigen (LCA), cytokeratin (CK), and anti-melanoma (MEL). Best results with reliable and specific immunostaining and a labelling int...